Orexin/hypocretin receptor chimaeras reveal structural features important for orexin peptide distinction
نویسندگان
چکیده
منابع مشابه
Orexin directly excites orexin neurons through orexin 2 receptor.
Orexin neurons (hypocretin neurons) have a critical role in the regulation of sleep/wakefulness, especially in the maintenance of arousal. Here, we revealed that orexin neurons are directly and indirectly activated by orexin via the orexin 2 receptor (OX2R). Orexin B (1 μM) induced depolarization in orexin neurons, which was still observed in the presence of TTX (1 μM), AP-5 (50 μM), and CNQX (...
متن کاملGenetically engineered immunoglobulins reveal structural features controlling segmental flexibility.
We have carried out nanosecond fluorescence polarization studies of genetically engineered immunoglobulins to determine the structural features controlling their segmental flexibility. The proteins studied were hybrids of a relatively rigid isotype (mouse IgG1) and a relatively flexible one (mouse IgG2a). They have identical light chains and heavy chain variable regions and have the same combin...
متن کاملMechanoenzymes under superstall and large assisting loads reveal structural features.
Single-molecule experiments on the motor protein kinesin have observed runs of backsteps and thus a negative, that is, reverse mean velocity, V, under superstall loads, F; but, counterintuitively, beyond stall, V(F) displays a shallow minimum and then decreases in magnitude. Conversely, under assisting loads V(F) rises to a maximum before decreasing monotonically. By contrast, while the velocit...
متن کاملLessons from MPO deficiency about functionally important structural features.
Genetic abnormalities often serve as the catalyst to stimulate critical insights into normal biology. In many cases, examining such experiments of Nature explicates not only the abnormal but also serves to illustrate underlying normal principles. Within the context of myeloperoxidase biosynthesis, we have examined the impact of specific missense mutations that cause inherited myeloperoxidase de...
متن کاملConfounders versus Mediators: An Important Distinction.
To the Editor: With some degree of amazement, we read the article by Kashy et al.1 on the influence of 6% hydroxyethyl starch (HES) 670/0.75 (Hextend; Hospira Inc., USA) on perioperative acute kidney injury in patients undergoing noncardiac surgery. The data are derived from a database of more than 120,000 patients treated in Cleveland hospitals, in which 6% HES 670/0.75 was the most commonly u...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 2011
ISSN: 0014-5793
DOI: 10.1016/j.febslet.2011.04.020